Pbabe hygro

pbabe hygro 果蝇细胞表达系统(Invitrogen):pAc5. 1/v5-his ac5forwardbgh pad-gal4-2. 1. The photomicrograph of H&E stained tissue sections of pBABE-Satb2 group (a) and pBABE-hygro group (b) 1 week after the implantation showed that newly formed bone could be observed 7 days after implantation, and activated cells were well organized around the implants. , 2002), pWZL-Blast-ST (plasmid # 13805) (Chen et al. Current Stock: pBABE-hygro-hTERT. , 2004), lentiviral packaging plasmid psPAX2 and envelope plasmid pMD2. The immortalized cell line POV71-hTERT was created by infecting POVs with viruses made containing the pBABE-hygro-hTERT plasmid . 2-GW EmGFP-miR pCMV-AD Description: Plasmid type: Mammalian two hybrid system Copy Number: High copy Promoter: CMV Size: 4. 1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg. The DREAM complex associates with the co-repressor Sin3B to repress target genes during quiescence. 9µg gag/pol expression vector, 0. Six hours after transfection cells were either stimulated with LPS (1 μg/mL) for 24 h or remained unstimulated. Register domain Name. rtTA was subcloned from Addgene 19780 into pBABE‐hygro. e r h y g r o B Avr I A r s I ' N a e I X c m I B s 0 1765 pBABE-Hygro t t t cut t cut r Uncut Xba I This gel depicts the NEB 1kb ladder and a digestion of the plasmid pBABE-Hygro with the following enzyme/enzymes: Xba I Men1 −/− immortalized MEFs were then infected with pBABE-Hygro alone, pBABE-Hygro-MEN1, or pBABE-Hygro expressing one of the seven MEN1 mutants. 65000. Louis, MO 63110-1093 pCMV-AD Description: Plasmid type: Mammalian two hybrid system Copy Number: High copy Promoter: CMV Size: 4. 免费电话:400-800-2947 010-53513060. Catalog number: RTV-001-HYGRO. pbabe-zeo 载体序列: origin. Peter Ratcliffe (Oxford University, UK). Cloning capacity = 5. The retroviral vector pBabe-PDGFR -Zeocin vector was constructed by inserting the fragment encoding PDGFR into the pBabe-Zeocin vector. pWZL Hygro TRF2ΔBΔM Addgene 18013 pBABE-puro Addgene 1764 pBABE-puro-TRF2ΔBΔM This paper - pBABE-puro-RasG12V Addgene 1768 Abstract. QUANTITY AND CONCENTRATION: 100 µL of bacterial glycerol stock . Mammary fibroblasts and microvascular endothelial cultures were prepared Terms and Conditions ©2004-2018 Harvard Medical School PlasmID was created and is maintained by the DF/HCC DNA Resource Core at Harvard Medical SchoolDF/HCC DNA Resource Human diploid fibroblast Tig3 cells expressing the ecotropic receptor, hTERT, and the (pBabe-hygro) empty vector, were transduced with pBabe-puro-BRAFE600 (OIS). To examine anchorage-independent growth, the p53 −/− astrocytes (1 × 10 4) infected with pBABE-Puro, pBABE-aurora B-Puro, pBABE-survivin-Hygro, and pBABE-aurora B-Puro + pBABE-survivin-Hygro were cultured into 6-well soft-agar dishes (1. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. com Creation Date: 2009-11-26 | 233 days left. 6% and 0. It can be applied to plap-1 gene function research in the future. hygro to form pJN105-hygro. pBABE-3*FLAG-APC Plasmid. 4 e, dark blue line). Cell lines carrying translocations represent important models to study the oncogenic properties of fusion proteins and develop therapeutic strategies. 查看联系方式>> pBABE ベクターは PMXs ベクター同様、哺乳類細胞に効率よく目的遺伝子を導入することができます。 RTV-001-HYGRO: 10 UG 我们拥有各种逆病毒载体,保证质量,请需要的朋友联系:i47-825o-882o,欢迎您光临本网站! COLORADO, USA — Gov. 8 during a briefing Friday on the state's response to LOCUS pBABE-hygro-hTERT Sequencing Result 8703 bp DNA SYNDEFINITION pBABE-hygro-hTERT Sequencing ResultACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. Virus production and cell lines. f(j15093) pbr322. To obtain the pBabe-hygro vector, the neomycin resistance gene of pBabe-puro was substituted with a 1. 106000. CDK4 R24C cDNA, provided by Thomas Wolfel (Johannes Gutenberg-Universitat, Germany) was ligated into the EcoRI site of pBabe-hygro. pBABE-Hygro载体载体序列 LOCUS Exported 5558 bp ds-DNA circular SYN 29-11-2013 DEFINITION Retroviral expression vector derived from the Moloney murine leukemia virus. f 3xfag. , 1986). 4) Do not vortex; swirl to mix. B, growth effects of constitutive p14/ARF expression in transfected DLD-1 cells. KRAS-overexpressing cells were obtained by retroviral infection with a pBABE-hygro KRAS4B construct and selected in hygromycin (800 mg/mL). D. 191-4401. Currently, there is no effective therapeutic strategy in the clinic for PTEN-deficient cancers. Abbreviations used to designate cell lines are as follows: J cells are infected with pBabe(puro)-v-Jun and pBabe(hygro), JM cells with pBabe(puro)-v-Jun and pBabe(hygro)-Marcks, JA cells with Mutations were introduced into full-length human mutant ERBB2 and subcloned into the pBABE-hygro vector using the QuikChange II XL Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA) and verified by sequencing. (b) A site of pBabe-hygro, pBabe-puro (22), or the blasticidin-resistant vector pWZL-Blast (gift of J. com, LLC with ip address 104. 1) was cloned into the retroviral vector pBABE-Hygro. 150 ul for a 6 well, 300 x 60 mm, 500 x 100 mm. r pbd-gal4 p1(j17588) p2(j17589) pbd-gal4 cam gal4bd t7 pbi121 35s(j3788) pbindt3 t7eev p53 is the most frequently mutated tumour-suppressor gene in human cancers. 121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt pBABE-3*FLAG-APC Plasmid. 2-fold), and cells with pBabe-puro H-rasV12 expressed the highest level of Ras (12-fold) (Fig. After the cells were cultured for 3 days, the mouse iPSCs were suspended at 2 × 10 7 cells/mL in PBS. 1-H1 neo载体 zt208 pSilencer 4. vector sequence - pBABE vector sequences (Jan/09/2004 ) Does anyone have the sequences of the pBABE series of retroviral vectors? I have a kinda sketchy plasmid map . 5ug) and pEGFP-N3 (0. (C) SA-β-gal staining of lamin B1↑lamin A/C↓ expressing pBABE-hygro-hTERT or pBABE-hygro (PH; phase contrast, BF; brightfield). Scale bar = 50 µm. (2014). 载体名称正向 反向 16srna 27f 1492r ez-t t7/m13f t3/m13r p3x-flag-cmv-7. 7% bottom and top agar, respectively) for 3 weeks. (B) IMR90 cells were infected by a retrovirus (pBabe-hygro-ER) encoding a murine ecotropic retroviral receptor (ER). f pbad30. Cells were selected in puromycin (Sigma-Aldrich) or hygromycin B (Thermo Fisher), and AXL knockdown was Yazdan Shahmorad A, Tian N, Kharazia V, Samaranch L, Kells A, Bringas J, et al. pMIG-hTERT was produced by introducing the hTERT cDNA from pCI-hTERT-neo (9) into pMIG. , 2003), and envelope plasmid pHCMV-AmphoEnv (plasmid The following retroviral vectors were used: pBabePuro-GSE56, expressing DN-p53 (Skeen et al. British Pound Eur; Polish Zloty US Dollar Voortstraat 49 1910 Kampenhout Belgium pBABE-Hygro Retroviral Vector Add to Cart. r pavu6+27 u6 pb42ad(pjg4-5)pb42ad-f pb42ad-r pbabe pbabe-f pbad pbad-f pbad-r pbad30 pbad30. , 2004); pBabe-Puro-FoxO(AAA)-ER, expressing FoxO1, with a triple mutation (T24A/S256A 名称 编号 规格 价格 手册; pBABE-Hygro: 60908- 7590: 2ug: 询价 132 4808 5519 021-57880797. TSC2 addback cell lines were generated by retroviral infection following transfection of PT67 cells with pBabe hygro IRES-EV or pBabe hygro IRES-TSC2. 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag pBABE-Hygro载体质粒图谱. 1990 Jun 25;18(12):3587-96. 2 | Virus transduction and selection Vector constructs used were pBabe-hTERT-hygro and pMESVts-neo,17,18 containing the telomerase catalytic subunit and a temperature-sensitive mutant of the SV40-Large T anti-gen (tsLT), respectively. ,2007), PKA catalytic subunit C obtained from pC EV (Addgene plasmid #15310) (Uhler and McKnight, 1987) and a DN mutant of the PKA RI With the strong CMV promoter upstream of an MCS and EF1α driving a hygromycin resistance marker, pCDH-CMV-MCS-EF1α-Hygro is a great, all-purpose lentivector Probably solved. Cells were selected with 400 μg/mL hygromycin B (Thermo, 10687010). But the absorbance of MTT test and the amount of HC were prior to UE at every measuring time and the amount of HC increased four times within 8 days (P < 0. pBabe-Puro GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 GENTAUR U. I was transfecting my cells using a mammalian expression vector pCMVSport6 which carries the gene of interest and a contransfect vector pBABE-Hygro which contains the selection marker. 2 GI:151108472 Species: Human Synonyms: CGI-135 (CANCERRESEARCH58. This plasmid is available through Addgene. 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt. 如果您有任何疑问或是问题,请随时与我们联系. , 2006); pLPCX-GFP and pLPCXGFP-DN-FoxO, expressing the amino acids 1-280 of FOXO1 (with Thr24 and Ser256 replaced with alanines) fused in frame with eGFP (Zhao et al. Virol, vol 70, p 5701), use 1µg transfer vector (pBabe, pMSCV-IRES-GFP, etc. 2 kb HindIII/BglII hygromycin resistance gene cassette from the pgk-hyg vector. The shape of growth curve of HC was similar to UE. 4 e, red line). Toggle menu. Plasmid pBABE-hygro p53 DD from Dr. . Cells were selected with hygromycin at 200 μg/ml for 1 week. pBABE-Neo Retroviral Vector . Fusion genes resulting from recurrent chromosomal translocations are associated with several tumor types. Cells were selected for 2 days with 500 mgyml hygromycin B, then several clones were isolated. Contains a hygromycin resistance marker. hygro-mCherry-GFP FIS 101-152(end) Full Snapgene Map: DU40799. r pbd-gal4 cam gal4bd t7 pbi121(35s) 35s pbind t3 t7eev pbk-cmv t7 t3 pbr322 pbr322. Welcome to Gentaur BVBA! Select Currency: EUR. If u have your protein fluorescent tagged, its even easier, because u can pick clones in between selection. 121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat pbabe-hygro-htert 载体质粒图谱和多克隆位点信息: pbabe-hygro-htert 载体序列: origin. If using low DNA concentration in water, make a stock of NaCl Primer Finder - bionicsro. r(j15094r) pbs(sk/ks) m13f/t7 pbabe-zeo 载体质粒图谱和多克隆位点信息: 载体简介. com Creation Date: 1999-05-07 | 7 years, 39 days left. 5ug) per well (6 well plate) LipoD293™ reagent (left panel) and Fugene HD (right panel) respectively per manufacturers' protocols. Plasmid pBABE-hygro from Dr. British Pound Eur; Polish Zloty US Dollar Voortstraat 49 1910 Kampenhout Belgium I am also looking for a pbabe hygro since the one that I have looks more like a pbabe puro tha a hygro I might use other retroviral vectors as pQCXIH QUOTE (Akhil Maheshwari @ Jan 7 2006, 08:32 PM) Mouse iPSCs were transduced with pBABE-hygro-Vector for 6 h. 175000. Welcome to Gentaur BVBA! Select Currency: EUR. Individual clones were analyzed for basal luciferase activity, and four independent clones were subsequently infected with either empty pBABE-puro vectororac-Myc2expressionvector. were cloned into pBABE-puro and pBABE-hygro backbones to generate retroviral transfer vectors. This plasmid is available through Addgene. pBabe(hygro)-GHR or pBabe(puro)-JAK2 was transiently cotransfected into 293T cells with pC/ECO to generate recombinant The following retroviral vectors were used: pBabePuro-GSE56, expressing DN-p53 (Skeen et al. 153000. 32 Briefly, cDNA for mCherry, GFP, and residues 101‐152 of mouse FIS1 were cloned into a pBABE. Selection with 1mg/ml puromycin was applied and resistant cells were pooled and designated as passage 1. 56. LinX cells were transfected with pBabe- or pFB-based vectors Although we obtained hTERT-deficient clones overexpressing LMNB1 and the control vector pBABE-hygro, these cells proliferated very poorly (Fig. 00: cDNA Clones : 14-3-3 epsilon View Data: pcDNA5-FRT/TO NRL-14-3-3 epsilon hi, we used for cell immortalization 2 viral vectors pBabe hygro hTert and pBabe neo Tsv40. Toggle menu. The construction of pBABE-hygro-hTERT (9), pBABE-puro-H-Ras (68), and pBABE-puro-HA-cyclin D1 (25) has been described previously. For making VSV-G retroviruses by triple transfection use 0. Toggle menu. C. Ret-roviral pBABE-Puro-hUSP27X-C-V5-FL-WT was obtained after BamHI/SalI subcloning from the corresponding pBABE-Zeo vec-tor (22). Y55F SPRY2 cDNA (Dr. Transfection and selection data of HeLa cells by various antibiotics in differing media. Genbank: Histomorphometric analysis of pBABE-hygro group and pBABE-Satb2 group. Margaret Chou, University of Pennsylvania) were subcloned into pBabe. 0载体 zt100 pSilencer5. I was transfecting my cells using a mammalian expression vector pCMVSport6 which carries the gene of interest and a contransfect vector pBABE-Hygro which contains the selection marker. 1( )hygro. Senescence was induced with doxycycline (D3447, Sigma-Aldrich) for the expression of B-RAF-V600E and with 4-hydroxy-tamoxifen (H6278, Sigma-Aldrich) for the expression of C-RAF and RASval12. C2C12 myoblasts stably expressing a functionally inert, tandem mCherry‐GFP tag fused to the mitochondrial targeting sequence of FIS1 were generated using the methods described by Allen et al. 477 /label="3MoMuLV_LTR" misc_feature 547 pBABE-Hygro逆转录病毒克隆载体: pBABE-Hygro Retroviral Vector: Cell Biolabs: RTV-001-HYGRO: 10ug: 逆转录表达载体种类较多,根据其基本载体骨架分为pBABE、pMCs、pMXs、pMYx等,又有不同筛选标记,hygro(潮霉素)、neo(新霉素)、puro(嘌呤霉素)、Blasticidin(杀稻瘟素)等。 zt205 pSilencer 2. The parent, “empty” pBABE and pLXSN vectors were used as controls. Plasmid pLB2 Ubi-FLIP FF CTV pBABE hygro MEN1 WT pAG305GAL-ccdB BioVector NTCC Inc. quantity: price: to the supplier. 1997). Read 19 answers by scientists to the question asked by Elamurugan Appavoo on Dec 29, 2014 Naviaux (1996) J. CAFs were immortalized for continual culturing by infecting with hTERT (pBABE-hygro-hTERT) within 2-weeks of CAF line establishment. The following viral plasmids were used: pBabe-puro (Morgenstern and Land 1990), MarXII-hygro, mouse c-myc/MarXII-hygro, mdm-2/MarXII-hygro (from Dr. They were nucleofected with linearized pBabe-hygro-hTert (Jeanblanc et al. More than 20 requests Plasmid pBabe-hygro PyMT from Dr. , 2012), then hygromycin B-resistant clones were trypsinized and amplified together to give an immortal cell population. . Box 8228 St. PCH‐2 regulates Caenorhabditis elegans lifespan. PEI complexes in NaCl, see transporter 5 protocol for buffer recipe. pBABE-hygro-hTERT: PVT17568: Lifescience Market: 2 ug: EUR 341: pBABE-Neo-hTERT Retroviral Vector: RTV-005: Cell Biolabs: 100 µL: EUR 717: Description: Use this gene pBabe-hygro-hTERT was constructed by inserting the EcoRI-to-SalI fragment containing the full-length hTERT cDNA from pCI-Neo-hTERT into the EcoRI–SalI sites of pBabe-hygro . Cuitiño et al. 5 µg/µL in TE Background Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. VERSION . 1 pflag-cmv-r /cmv24 pact t7 t3/ebv-r pact2 gal4ad pact2-r/3'ad pact5. hygro vector kindly provided by Dr Ian Ganley. Lékařská biotechnologická výzkumná laboratoř Home; suppliers; Zeptometrix NatTrols; Distributors; FAQ; mtDNA Sequences; mitochondrial membrane The study of E2Fs in vivo has been challenging. 158000. Pbabe vector map. 175000. et al. Ganley (University of Dundee, Dundee, Scotland, UK) through the Medical Research Council Protein Phosphorylation and Ubiquitylation Unit Reagents and Services facility (College of Life Sciences, University of Dundee, Scotland; pBabe. After infection of pBABE-hygro or pBh-FOXO4, the cells were selected with Agilent technologies pbabe hygro flag mlh1 n terminus mutant Pbabe Hygro Flag Mlh1 N Terminus Mutant, supplied by Agilent technologies, used in various techniques. Widespread optogenetic expression in macaque cortex obtained with MR-guided, convection enhanced delivery (CED) of AAV vector to the thalamus. Transfection and selection data related to pBABE-puro in cell-culture. Bainor et al. 1-H1 hygro载体 zt207 pSilencer 3. Wild-type HIF-1 and HIF-1 P402A,P564G expression plasmids were a kind gift from Dr. reconstruct the spatiotemporal expression of E2F activators (E2F3A) and canonical (E2F4) and atypical (E2F8) repressors during the mammalian cell cycle and propose that orchestrated accumulation of different E2F combinations control gene expression in proliferating (E2F3A-8-4) and differentiating (E2F3A-4) cells. hTERT expression constructs lacking the HA tag were created by PCR of an hTERT EcoRV–SalI fragment with an oligonucleotide that deleted the nucleotides encoding the HA tag. Gary Nolan, Stanford University, Stanford, CA). store at supplier with ip address 188. 89000. ZERO BIAS - scores, article reviews, protocol conditions and more pBabe. We meet 2 problems: For TAZ S89A overexpression, TAZ was amplified from the pBABE-hygro-hTAZ construct made in-house with the restriction sites, BamH I and Xho I, and subcloned into the pLenti-CMV-GFP-puro plasmid (a gift from Eric Campeau & Paul Kaufman, Addgene plasmid #17448) digested by restriction enzymes, Bam HI and Sal I. NF2/Merlin plasmid is Addgene 19699. Cell number was assayed by MTT after seeding for 1, 3, or 5 days. 1-U6 hygro载体 zt206 pSilencer 3. British Pound Eur; Polish Zloty US Dollar Voortstraat 49 1910 Kampenhout Belgium the viruses: pBABE‐MDER (gift from Stephen Tapscott; Addgene plasmid #13494), pBABE‐neo‐hTERT (gift from Bob Weinberg; Addgene plasmid #1774), pBABE‐hygro CDK4 R24C (gift from Bob Weinberg; Addgene plasmid #11254) and pBABE puro cyclinD1 HA (gift from William Hahn; Addgene plasmid #9050). Expression was confirmed by immunoblotting. Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Recombi-nant retroviral particles were generated using the Platinum pBABE-Hygro Retroviral Vector from Cell Biolabs. found that PTEN-deficient cells and cancers are uniquely sensitive to PDHK1 inhibition and propose PDHK1 as a potential therapeutic target in PTEN-deficient cancers. Supported by Natural Science Foundation of Shandong Province (Grant No. 1 gal4ad pad-gal4-2. In general, lentivirus and retroviruses were produced by transienttransfection of HEK293Tor293TPhoenix gag-polcells, into BamHI and NotI restriction sites of pcDNA3. 2kb Resistance(s): Ampicillin (Amp) pbabe-hygro 载体质粒图谱和多克隆位点信息: pbabe-hygro 载体简介. 175000. 1 µg VSV-G expression vector, and 1 µg transfer vector. Establishment and Transduction of Primary Cell Cultures. 1997) and selecting for G418-resistant cells. TEFLY-A colonies transduced with pBabe-hygro-hTERT, and therefore resistant to hygromycin B, were isolated and expanded, and a clone producing high-titer full-length pBabe-hygro-hTERT was selected. 238. pBabe hygro HA RFP (dsRED) TMEM115 M1-P229 Parent Plasmid: pBABE hygro DU Number: DU62297 Species: Human Synonyms: Sequence of Insert: The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. While pBABE Puro caused PML and HSP70 coaggregation in transfected cells (as shown in Figs 1, 2, 3), none of the other pBABE vectors had this effect (Fig 4A). Chatterjee et al. [Construction and confirmation of a recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1] pBabe. , 2005), pBabe-neo-large T cDNA (plasmid # 1780) (Hahn et al. Morgenstern, Millen-nium Pharmaceutical, Cambridge, MA). Infected cell populations were selected in blastcidin (2. pBABE-Hygro-hTERT Retroviral Vector: 100 ul: RTV-005: pBABE-Neo-hTERT Retroviral Vector: 100 ul: RTV-006: pBABE-Puro-hTERT Retroviral Vector: 100 ul: Retrovirus quantitation kit: VPK-120: QuickTiter Retrovirus Quantitation Kit: 20 assays: Retrovirus transduction kits: RV-200: ViraDuctin Retrovirus Transduction Kit (40 Transductions) 40 The retroviral expression constructs, pBabe-Zeo-SV40ER, pBabe-Hygro-hTERT,andpBABE-puro-ras-V12werekindlyprovidedbyDr. pBabe-Puro-MEK-DD(Addgeneplasmid#15268)(Boehmetal. The following plasmids were used for the generation of retroviruses: pBabe (Morgenstern and Land, 1990), pLPC (the gene of interest is expressed from the CMV promoter and the gene for puromycin resistance is driven by an LTR), pWZL (co-expresses the gene of interest with a selectable marker translated from an internal ribosomal entry site) and pZIPneo (Jat et al. After the ligation of IRF-3 or IRF-7 cDNA fragments into the BamHI site of pET15b (Novagen), His-tagged IRF-3 and IRF-7 cDNA fragments were prepared by EcoRV/XbaI Finally, the recombinant plasmid pBABE-hygro-PLAP-1 was confirmed by agarose gel electrophoresis and DNA sequence analysis. To produce replication-incompetent lentivirus, 293T cells were co-transfected with mCherry pUltra-hot (Addgene #24130) construct in combination with REV, VSVG, PDML (Addgene) using Lipofectamine Plus reagent This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. 2. pBabe hygro FLAG-mCherry 14-3-3 epsilon : Human : FLAG, mCherry : Mammalian : £110. 4. We have therefore generated `knock-in pCMV-AD Description: Plasmid type: Mammalian two hybrid system Copy Number: High copy Promoter: CMV Size: 4. pBABE-hygro载体质粒价格1250元,pBABE-hygro载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为氨苄青霉素,质粒大小为5578BP,测序引物见下文。 pBABE-Hygro Retroviral Vector: RTV-001-HYGRO-CB: 10 ug: 535 € DETAILS Add to Cart pBABE-Hygro-hTERT Retroviral Vector: RTV-007-GVO-CB: 100 ul: 672 € DETAILS Add to Cart pBABE-Neo Retroviral Vector: RTV-003-GVO-CB: 100 ul: 535 € DETAILS Add to Cart pBABE-Neo-hTERT Retroviral Vector: RTV-005-GVO-CB: 100 ul: 672 € DETAILS Add to Cart GM07166 cells were immortalized by infection with a retrovirus (pBabe/hygro) encoding hTERT (Meyerson et al. 71. For generating stable cell lines expressing FOXO4, we constructed the retroviral vector pBABE-hygro (pBh)-FOXO4. pBABE-hygro NM23 H1 His pLB2 CAG P2Gm pCS2 mt-GFP (BamHI minus) pBABE hygro MEN1 A309P pAG303GPD-ccdB-EGFP. , 2006); pLPCX-GFP and pLPCXGFP-DN-FoxO, expressing the amino acids 1-280 of FOXO1 (with Thr24 and Ser256 replaced with alanines) fused in frame with eGFP (Zhao et al. Cells were transfected with Fugene 6 (Roche) following the manufacturer’s recommendations. pBABE-Hygro-hTERT Retroviral Vector: RTV-007 : Cell Biolabs: 100 µL: Add: pMXs Retroviral Vector: RTV-010 : Cell Biolabs: 10 µg: Add: pMXs-Neo Retroviral Vector Hygro (HIII-Cla cassette from pBabe Hygro) Blast (HIII-Cla blasticidin cassette by PCR from ) GFP (EcoRI fragment blunt in HIII-NsiI) YFP (PGK/-Xba-AgeI-NcoI-/YFP/-Bam-Spe-Xba, no Cla left) mRFP (PGK/-Xba-/mRFP, no Cla left) empty vector contains no cassette but intact polylinker Clone in a pSupercassette with XhoI and EcoRI CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The following plasmids were used for the generation of retroviruses: pBabe (Morgenstern and Land, 1990), pLPC (the gene of interest is expressed from the CMV promoter and the gene for puromycin resistance is driven by an LTR), pWZL (co-expresses the gene of interest with a selectable marker translated from an internal DLD-1 cells were stably transfected with vectors pWZL-hygro, pWZL-hygro-p14, pBabe-puro-EGFP, and pBabe-puro-p16 as described in Materials and Methods. Register domain TLDS L. Compare Search ( Please select at least 2 keywords ) Most Searched Keywords. 175000. Retroviral particles were produced using the ecotropic Phoenix packaging cell line with the following plasmids: pWZL-hygro; pBABE-puro; pWZL-hygro 12S E1A or pBABE-puro K-Ras V12 (purchased from Addgene, Cambridge, MA). FEATURES Location/Qualifiers source 1. The construct was co‐transfected into HEK293 FT cells with GAG/POL and VSV‐G expression plasmids (Clontech, Saint‐ Germain‐en‐Laye, France) for Retroviral vectors (pBabe-blast, -neo and -puro) were used to introduce the SV40 ER, c-Myc and H-ras into TIG-3 cells, respectively. com, Inc. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. The LNCap cells were grown as ATCC recommended procedures and co-transfected with pBabe-hygro-SSeCKs (1. This plasmid is available through Addgene. 5 μg This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. Growth curves A total of 5 104 cells were initially seeded (cell input=n 0) In honor of a father, pBABE gets its name It so happened that the genesis of the vectors coincided with the 60 th birthday of my father, Harold Morgenstern, better known by his nickname of “Babe” (he was the youngest in the family and he was born when the legendary baseball player Babe Ruth was in his prime). G (plasmid #12260, #12259), retroviral packaging plasmid pUMVC3 (plasmid # 8449) (Stewart et al. pBABE-hygro BMSCs and the (d) pBABE-Satb2 BMSCs. British Pound Eur; Polish Zloty US Dollar Voortstraat 49 1910 Kampenhout Belgium pBabe-Hygromycin, pBabe-Zeocin, and pBabe-DNp53-Puromycin ex-pressing a dominant-negative p53 (DNp53), due to a point mutation of p53R175H affectingtheDNAbindingability,werekindlyprovidedbythe lab of Dr. 61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata. pBABE-hygro- SLC1A3 was sequenced to confirm that no mutations arose during the cloning. pbabe-hygro 载体序列: origin. Euclid Ave. Cell Biolabs’ retrovirus vector is based on the pBABE vector system, which is derived from Moloney murine pBABE-Hygro Retroviral Vector: RTV-001-HYGRO-CB: 10 ug: 535 € DETAILS Add to Cart pBABE-Hygro-hTERT Retroviral Vector: RTV-007-GVO-CB: Glycerol Stock: 672 € DETAILS Add to Cart pBABE-Neo Retroviral Vector: RTV-003-GVO-CB: Glycerol Stock: 535 € DETAILS Add to Cart pBABE-Neo-hTERT Retroviral Vector: RTV-005-GVO-CB: Glycerol Stock: 672 To generate DISP3-overexpressing NS-5 cells, the full-length human DISP3 (GenBank AB037758. LOUIS Department of Cell Biology and Physiology 660 S. 5-fold above the endogenous Ras level), cells with pBabe-zeo H-rasV12 expressed intermediate levels (7. 7. NEDD9 is an integrin signaling adaptor protein composed of multiple domains USA - ST. View Parent Plasmid | DU Number: DU40799. CATALOG NUMBER: RTV-003 STORAGE: -80ºC . Dafna Bar-Sagi, New York University, New York, NY) and MKK3 cDNA (Dr. 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca. This suggested that the puromycin resistance gene was responsible for PML and HSP70 aggregation. Sun, CSHL), E6/pBabe-puro, cdc25A/MarXII-hygro, cyclin D1/pBabe-puro, rasV12/pBabe-puro, E1A/pWzl-hygro, p53175H/pWzl-hygro, cdc25C/pBabe-puro, and E7/pBabe-puro. Cell pBABE-Hygro-3 Plasmid Rating * Name Review Subject * Comments * €258. We use electroporation to transfert these ones into empackating cell (PT67). A growing body of evidence has shown the signaling crosstalk between EGFR and integrins in cellular migration and invasion. pBABE-Hygro Retroviral Expression Vector. 477 /label="5_LTR2" misc_feature 16. pcDNA Htt25Q-mCFP and Htt103Q-mCFP (29) were kindly provided by Ai Yamamoto (Columbia University) and sub-cloned into pBABE-hygro (Addgene plasmid 1765). 8703 /organism="pBABE-hygro-hTERT Sequencing Result" /mol_type="other DNA" misc_feature 10. Cargo net replacement hooks 1 . com 载体名称正向 反向 16srna 27f 1492r ez-t t7/m13f t3/m13r p3x-flag-cmv-7. Clone your gene of interest into one of these vectors and transfect into a retroviral packaging cell line, 293T, or 293RTV cells. Email:[email protected] 2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6. dna. hygro. Amphotropic retroviruses were produced by Abbreviations used to designate cell lines are as follows: J cells are infected with pBabe(puro)-v-Jun and pBabe(hygro), JM cells with pBabe(puro)-v-Jun and pBabe(hygro)-Marcks, JA cells with GHR cDNA was generated from mouse liver by RT-PCR, confirmed by DNA sequencing, and subcloned into a retroviral vector [pBabe(hygro)-GHR]. The desmoplastic small round cell tumor is a rare sarcoma characterized by translocation between the EWSR1 and WT1 genes, resulting in Pbhab. Mutant p53 is thought to contribute to carcinogenesis by the acquisition of gain-of-function properties or through the exertion of dominant-negative (DN) effects over the remaining wild-type protein. 05). P. Hartmut Land's lab is published in Nucleic Acids Res. pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro Lentiviral vector for barcoded shRNA libraries, with an HTS4 cassette for high-throughput sequencing plus TagRFP and puromycin resistance markers. Parent Plasmid: pBabe hygro mCherry GFP. RESULTS: The construction of recombinant eukaryotic expression plasmid pBABE-hygro-PLAP-1 was confirmed through restriction enzyme maping analysis and DNA sequencing. pBABE (hygro) mEOS2 hYAP 1-2gamma Addgene catalog: 124161. Cell death analysis. Pbabe puro vector. 2kb Resistance(s): Ampicillin (Amp) gibco胎牛血清 hyclone胎牛血清 sigma胎牛血清 corning胎牛血清 bi胎牛血清 gemini胎牛血清 pan胎牛血清 bovogen胎牛血清 hyclone澳洲胎牛 flir フリアー サーモグラフィカメラ c5。flir c5 フリアーシステムズ 3. . d/b/a SRSPlus store at supplier GoDaddy. hygro-mCherry-GFP FIS 101–152(end) [DU40799 IFN regulatory factor 3 (IRF3) is a transcriptional factor that plays a crucial role in activation of innate immunity and inflammation in response to viral infection, and is also involved in p53-dependent inhibition of cell growth. 2kb Resistance(s): Ampicillin (Amp) (B) Growth curve of lamin B1↑lamin A/C↓ cells expressing either pBABE-hygro-hTERT or pBABE-hygro control. More than 20 requests pBABE-Hygro Retroviral Vector CATALOG NUMBER: RTV-001-HYGRO STORAGE: -20ºC QUANTITY AND CONCENTRATION: 10 µg at 0. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5 ends. Control transfections were performed with retroviral vec- If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. AFter 10days of hygromycin selection, I did a pooling of the cell colonies left and checked on the overexpression of protein of interest. Bioz Stars score: 91/100, based on 1 PubMed citations. Obviously u will Pbabes. Toggle menu. RobertWeinberg pbabe–hygro or pbabe–hygro–Miz1 retroviruses. Jared Polis (D-Colorado) announced Coloradans age 65-69, as well as educators, will start to receive the vaccine Feb. 1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg. L. Four days after transduction, cells were harvested in Trizol. co. 61 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc. Bob Weinberg's lab contains the insert hTERT and is published in Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8. pBABE (hygro) mEOS2 hYAP 1-2delta Addgene catalog: 124162. 175000 Purpose: In a substantial population of non–small cell lung cancer (NSCLC), expression and activation of EGF receptor (EGFR) have been reported and is regarded as a novel molecular target. Although functional activation of IRF3 by viral infection is relatively well documented, the biological role and regulatory mechanism underlying cell growth Transfection and selection data of 293T cells by various antibiotics in differing media. The vector contains the human IL-6 promoter region upstream of the luciferase-encoding sequence of plasmid pBABE hygro. 89000. Plasmid pBABE-hygro-hTERT from Dr. Addgene 0 遺伝子特異的リコンビナント レトロウイルスベクター | レトロウイルスは、分化細胞のゲノムに遺伝子を導入するのに効果的なツールとして用いられています。 In 8/10 cases I got desired clones with pBABE-puro co-transfection. f pbad30. Cellswereselectedin1 mgyml Cell Biolabs' pBABE vectors are suitable for making retrovirus for general gene expression in various cellsIn molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The photomicrograph of H&E stained tissue sections of pBABE-Satb2 group (a) and pBABE-hygro group (b) 1 week after the implantation showed that newly formed bone could be observed 7 days after implantation, and activated cells were well organized around the implants. 1-H1-Retro载体 zt099 pSilencer5. 3). 97 The tumor suppressor PTEN is widely inactivated in cancers and tumor syndromes. ( f ) Histomorphometric analysis showed that both the percentage of newly formed bone area and bone-to-implant contact increased by about 20 % in pBABE(cdk4R)hygro, which expresses a p16-resistant point mutant (R24C) form of cdk4 (61, 74); and pBABE(hTERT)puro, which expresses the catalytic subunit of human telomerase (17, 24). Filippo Giancotti's lab contains the insert Polyoma Middle T and is published in J Clin Invest. Similarly, murine JAK2 cDNA was inserted into a retroviral vector [pBabe(puro)-JAK2]. However, the context in which the DN effects are observed is not well understood. 1 PTEN pCM433 pBABE hygro MEN1 A242V pAG303GAL-ccdB-EGFP. Lentiviral-compatible shRNAs against Beclin 1 (RMM4431-101259746, sense: ACAGGAGCTGGAAGATGTGGAA; RHS4430-98520944, sense: AGCCAATAAGATGGGTCTGAAA) and vimentin (RHS3979-9596530, sense: 1 pBabe-hygro PyMT Plasmid: PVT14778: Lifescience Market: 2 ug: EUR 325: pBabe-HA-ER-IPpoI: PVT17437: Lifescience Market: 2 ug: EUR 300: Color Switch, CRE report cell Histomorphometric analysis of pBABE-hygro group and pBABE-Satb2 group. but would like to sequence my clones to check its the right thing but don't know the sequence of the backbone. pBABE-Hygro Retroviral Vector Map Recent Product Citation Qian, H. 1 V5-His B,pCo hygro 逆病毒载体(addgene )pLVTH,pLNCX2,pQCXIH,pMSCVpuro Gateway系统(invitrogen)pENTR3C, pcDNA6. 00) SKU: PVT17647. 119(2):252-66. BamHI and SmaI and inserted into pBABE-neo. Cat#: RTV-001-HYGRO-CB: Quantity: 10 ug: Price: 535 € Figure 1. The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. The infected population was selected using puromycin 1μg/ml. hygro-mCherry-GFP FIS 101-152(end) Parent Plasmid: pBabe hygro mCherry GFP DU Number: DU40799 Genbank: NM_016068. 2R2010HM050). Welcome to Gentaur BVBA! Select Currency: EUR. ) and 1 µg of the pCL vector. 2009 Feb . 154000. A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123 Tel (408) 780-0908, Fax (408) 780-0908, [email protected] A plasmid containing the mito-QC sequence was obtained from I. HMLER cells infected with pBabe-hygro H-rasV12 expressed the lowest level of oncogenic Ras (3. 89000. 175000. 184 Pbabe hygro map. 6 kb pBABE-Hygro Retroviral expression vector derived from the Moloney murine leukemia virus. The infected population was selected using puromycin 1μg/ml. Vectors may be Genomic DNA surrounded the viral replication origin may be amplified when the viral origin fires multiple times in a given cell cycle. Antibodies Stable expression of genes was achieved by transduction with retroviral expression vectors pBABE-hygro (for SV40 Small T Antigen), pRetroSuper (for shRNA against p53) or pBABE-puro (for all forms of RAS) 28 , 29 with viruses generated via calcium phosphate transfection, 30 followed by stable selection using 20 mug/ml hygromycin for pBABE-hygro (Invitrogen, Carlsbad, CA, USA), 5 mug/ml blasticidin for pRetroSuper (Sigma Aldrich, St Louis, MO, USA), and 5 mug/ml puromycin (Sigma Aldrich) for pBABE-3*FLAG-APC Plasmid. kr Vector ): pBABE-hygro vector was digested with BamHI and SalI and cDNA coding for human SLC1A3 (purchased from IDT) cloned using In-Fusion HD cloning kit from Clontech (Takara Bio USA, Inc). 166. 1-U6-Retro载体 zt535 psiLV-H1 zt457 pSIM5载体 zt458 pSIM6载体 zt2156 pSin-EF2-puro -Nanog pBABE-hygro NM23 H1 WT MSCV FLIPi P2G_Thy1. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. 175000. Warranty These products are warranted to perform as described in their labeling and in Cell Biolabs literature when used in accordance The recombinant plasmid of pBABE-hygro-PLAP-1 is successfully constructed. The vector also contained an SV40 replication origin. For all constructs, retrovirus was produced by calcium phosphate–mediated transfection of amphotropic Phoenix cells (Dr. This plasmid is available through Addgene. 175000. British Pound Eur; Polish Zloty US Dollar Voortstraat 49 1910 Kampenhout Belgium pBABE-hygro-hTERT载体质粒价格1500元,pBABE-hygro-hTERT载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为氨苄青霉素,质粒大小为8703BP,测序引物见下文。 pBabe-hygro PyMT质粒价格1200元,pBabe-hygro PyMT(爱迪基因#22305 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为氨苄青霉素,质粒大小为5100 bp,测序引物为pBabe 5'。 商品「pBABE-Hygro Retroviral Vector (CBL-RTV001HYGRO-10UG)(カテゴリ:遺伝子工学)」に関する情報をご覧いただけます。 pBABE-Hygro-hTERT Retroviral Vector: 中文名称: pBABE-Hygro-hTERT逆转录病毒克隆载体: 供应商: Cell Biolabs: 产品货号: RTV-007: 产品报价 ¥100uL: 购买方式: 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868。 产品新闻: 背景资料: 待更新 pBABE-Hygro Retroviral Vector: 中文名称: pBABE-Hygro逆转录病毒克隆载体: 供应商: Cell Biolabs: 产品货号: RTV-001-HYGRO: 产品报价 ¥10ug: 购买方式: 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868。 产品新闻: 背景资料: 待更新: 产品描述 The following plasmids were obtained from Addgene: pBabe-hygro-hTERT (plasmid # 1773) (Counter et al. r pact t7 t3/ebv-r pact2 gal4ad pact2-r/3'ad pas2-1 gal4bd(p24990) pavu6+27 u6 pb42ad(pjg4-5) pb42ad-f pb42ad-r pbabe pbabe-f pbad pbad-f pbad-r pbad30 pbad30. Welcome to Gentaur BVBA! Select Currency: EUR. 89000. 5インチタッチパネルスクリーン wifi機能付きコンパクトサーモグラフィカメラ フリアーc5 PMZX-ERMP80FEYのエアコンが激安価格!業務用エアコンの空調センター EX。6,000種類以上の豊富な品揃えとスピード対応でお客様の満足にお答えします。【関連キーワード】業務用エアコン 三相200V 天井埋込カセット形1方向 3馬力 ワイヤード<N800 同時ツイン 検索用ID:D2-103> . Gentaur Cz. S. Availability: Usually shipped in 5 working days. 1 3xfag. CONCLUSION: The transfection of pBABE-HYGRO-hTERT had greatly improved the proliferating abilities and activated the telomerase of UE 5hTERT with retroviral particles carrying pBABE-Hygro-T-Ag and subsequently after selection with 150mg/ml hygromycin for 7 days with packaged pBABE-Puro-H-RASV12G. 1-CMV neo载体 zt209 pSilencer 4. Contains a hygromycin resistance marker. , 1998), pBabe-HcRed-Ras (plasmid # 10678) (Boehm et al. October1. 175000. We obtained several transfected PT67 clones with antibiotics selection. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206. For retroviral expression, pBABE-puro constructs encoding ErbB4 JM-a Doxycycline‐inducible reporter systems were obtained by subcloning the tet‐responsive element from FudeltaGW plasmid upstream of the promoter‐luciferase elements into a puromycin‐resistant retroviral backbone. citations: 1. Drug selection was performed with 50 lg/ml neomycin (Invitrogen, Breda, The Netherlands). Retroviruses were prepared by transfecting the pBABE-3*FLAG-APC Plasmid. show that Sin3B inactivation is insufficient to allow S-phase re-entry but poises quiescent cells to proliferate upon inhibition of the APC/CCDH1 complex, highlighting a functional cooperation between transcriptional and post-translational cell-cycle regulation. Transductions and selection were performed essentially as described previ-ously (17, 58). The cloned ORF contains silent mutations to generate an siRNA resistant mRNA. Retroviral pBABE-Snail1-HA was previously described (12). Our pBABE vectors are suitable for making retrovirus for general gene expression in various cells. Size: 2 ug. Hartmut Land. 70000. Hecht Viral particles were produced in 293T cells, and target cells were incubated with viral media for 24 hours. 112000. type MET, or human METex14 in a pBABE-puro vector backbone as described previously (9) and selected in puromycin (2 mg/mL). ACCESSION . , 2004); pBabe-Puro-FoxO(AAA)-ER, expressing FoxO1, with a triple mutation (T24A/S256A LC3/RG cells were transiently transduced with pBABE-hygro Htt25Q-mCFP or pBABE-hygro Htt103Q-mCFP particles. Bob Weinberg's lab contains the insert p53 DD and is published in Mol Cell Biol 2002 Apr;22(7):2111-23. Order directly at Bio-Connect Wednesday, April 2, 2014 2:48 PM Page 1 of 1 pBABE hygro lucIL6 A m p R 3 M o M L T R l u c i f e r a s e I L 6 3 ' U T R S V . Packing: 10 ug. 1998] RetroviralTransferofHumanCytochromeP450GenesforOxazaphosphorine-basedCancerGeneTherapy1 YoussefJounaidi,JodiE. As expected, the same cell line with normal LMNB1 levels grew significantly better (Fig. 175000. U2OS cells that stably express SV40 T [U2OS(T)] were generated by transfecting U2OS cells with CMV T neo (Campbell et al. Cells were selected for 10 days in hygromycin and puromycin, and Giemsa stained thereafter. AFter 10days of hygromycin selection, I did a pooling of the cell colonies left and checked on the overexpression of protein of interest. Welcome to Gentaur BVBA! Select Currency: EUR. Toggle menu. 1-CMV puro载体 zt204 pSilencer1. This plasmid was transfected into TEFLY-RD cells (a packaging cell line producing envelope proteins derived from feline endogenous retrovirus RD114) by using calcium Human diploid fibroblast Tig3 cells expressing the ecotropic receptor, hTERT, and the (pBabe-hygro) empty vector, were transduced with empty vector (cycling). Aging. pBABE-hygro-hTERT Uncut 1kb Ladder This gel depicts the NEB 1kb ladder and a digestion of the plasmid pBABE-hygro-hTERT with the following enzymes: EcoRI-HF, SalI-HF and EcoRI-HF/SalI-HF double digest DNA digested with EcoRI-HF, SalI-HF and EcoRI-HF/SalI-HF double digest for 2 hours pBABE-hygro. pbabe hygro


Pbabe hygro